44174

edited by Tim D. Hewitson, Ian A. Darby.

New York, N.Y. Humana Press

x, 229 p. : ill. ; 26 cm

Springer protocols , Methods in molecular biology 1940-6029 611

Includes bibliographical references and index , Tissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmouli�ere Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld Lectin histochemistry for light and electron microscopy / Su Ee Wong ... [et al.] , Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong Special stains for extracellular matrix / Andr�ea Monte-Alto-Costa and Lu�is Crist�ov�ao Porto Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas M�oller In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing ... [et al.] High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas M�oller Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / J�org Piper Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.

AU Hewitson, Tim D , AU Darby, Ian A , SE Springer protocols , SE Methods in molecular biology (Clifton, N.J.) v. 611.

QM

556

.H575

2010

2009939928